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MU30369小鼠白细胞介素1β(IL-1β) Mouse Interleukin 1β(IL-1β)ELISA Kit

价格:    2400.00元

  • Catalog:    MU30369
96T

库存 100 件

MU30369小鼠白细胞介素1β(IL-1β) Mouse Interleukin 1β(IL-1β)ELISA Kit

2400.00元

Catalog : MU30369
用途

本试剂盒用于体外定量检测小鼠血清、血浆、组织、细胞上清及相关液体样本中白细胞介素1β(IL-1 β)的含量。

别称:

IL1b; IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin


检测原理

本试剂盒应用双抗体夹心法测定标本中小鼠白细胞介素1β(IL-1β)水平。用纯化的小鼠白细 胞介素1β(IL-1β)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素1 β(IL-1β),再与HRP标记的白细胞介素1β(IL-1β)抗体结合,形成抗体-抗原-酶标抗体复合 物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终 的黄色。颜色的深浅和样品中的白细胞介素1β(IL-1β)呈正相关。用酶标仪在450nm波长下测定 吸光度(OD值),通过标准曲线计算样品中小鼠白细胞介素1β(IL-1β)浓度。

 

反应类型

夹心法

反应时间

2h

反应

小鼠 

检测方法

Colormetric

检测范围

12pg/ml-960pg/ml

灵敏度

≤2.4pg/m

样本体积

50μL

样本类型

血清、血浆或其他相关生物液体


特异性

可检测样本中的小鼠白细胞介素1β(IL-1β),且与其它相关蛋白无明显交叉反应


重复性

板内变异系数≤9%,板间变异系数≤11%


试剂盒组成及保存
规格
96T

说明书

1份

封板膜

2张

酶标包被板

12孔×8条

标准 1920pg/ml

0.6ml×1 瓶

标准品稀释液

2ml×1 瓶

生物素标记的抗IL-1β抗体

1.5ml×1瓶

显色剂A液

6ml×1瓶

显色剂B液

6ml×1瓶

终止液

6ml×1瓶

酶标试剂

6ml×1瓶

浓缩洗涤液

20ml×1瓶(30倍)


保存温度:2-8°C 

 

实验室所需物品

需要而未提供的试剂和器材

1.吸光度值在450nm波长下检测的酶标仪

2.1-2ml的加样器

3.100 ml和1L的量筒

4.吸水纸

5.37°C 恒温箱

6.蒸馏水或去离子水

7.数据分析和绘图软件,图纸

8.标准和样品稀释的试管‍

 

操作步骤


 

1529560202828115.jpg



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