本试剂盒用于体外定量检测小鼠血清、血浆、组织、细胞上清及相关液体样本中白细胞介素1β(IL-1 β)的含量。
别称:
IL1b; IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
本试剂盒应用双抗体夹心法测定标本中小鼠白细胞介素1β(IL-1β)水平。用纯化的小鼠白细 胞介素1β(IL-1β)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素1 β(IL-1β),再与HRP标记的白细胞介素1β(IL-1β)抗体结合,形成抗体-抗原-酶标抗体复合 物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终 的黄色。颜色的深浅和样品中的白细胞介素1β(IL-1β)呈正相关。用酶标仪在450nm波长下测定 吸光度(OD值),通过标准曲线计算样品中小鼠白细胞介素1β(IL-1β)浓度。
反应类型 | 夹心法 | |||
反应时间 | 2h | |||
反应性 | 小鼠 | |||
检测方法 | Colormetric | |||
检测范围 | 12pg/ml-960pg/ml | |||
灵敏度 | ≤2.4pg/m | |||
样本体积 | 50μL | |||
样本类型 | 血清、血浆或其他相关生物液体 |
可检测样本中的小鼠白细胞介素1β(IL-1β),且与其它相关蛋白无明显交叉反应
板内变异系数≤9%,板间变异系数≤11%
规格 | 96T | ||
说明书 | 1份 | ||
封板膜 | 2张 | ||
酶标包被板 | 12孔×8条 | ||
标准 1920pg/ml | 0.6ml×1 瓶 | ||
标准品稀释液 | 2ml×1 瓶 | ||
生物素标记的抗IL-1β抗体 | 1.5ml×1瓶 | ||
显色剂A液 | 6ml×1瓶 | ||
显色剂B液 | 6ml×1瓶 | ||
终止液 | 6ml×1瓶 | ||
酶标试剂 | 6ml×1瓶 | ||
浓缩洗涤液 | 20ml×1瓶(30倍) |
保存温度:2-8°C
需要而未提供的试剂和器材
1.吸光度值在450nm波长下检测的酶标仪
2.1-2ml的加样器
3.100 ml和1L的量筒
4.吸水纸
5.37°C 恒温箱
6.蒸馏水或去离子水
7.数据分析和绘图软件,图纸
8.标准和样品稀释的试管
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