欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

PAB34937 RFX5 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34937
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human

库存 800 件

PAB34937 RFX5 Polyclonal Antibody

320.00元

Catalog : PAB34937
Application : WB,IF,ELISA,IHC
Reactivity : Human
Product Information


Product name

RFX5 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human RFX5

Calculated molecular weight

60kDa/65kDa

Observed molecular weight 

Refer to figures

Swiss Prot

P48382

Gene ID (NCBI)

5993

Alternative Names

DNA binding protein RFX5, Regulatory factor X 5, RFX5


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:100

IHC

1:50 - 1:500

 

       1                    

Immunofluorescence analysis of U2OS cells using RFX5 antibody.

 

Background


A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.                                                                            

                                                                                              

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.