欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

PAB34936 RBBP7 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34936
  • Application:    WB,IF,IP,ChIP
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB34936 RBBP7 Polyclonal Antibody

320.00元

Catalog : PAB34936
Application : WB,IF,IP,ChIP
Reactivity : Human,Mouse,Rat
Product Information


Product name

RBBP7 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,IP,ChIP

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human RBBP7

Calculated molecular weight

47kDa/52kDa

Observed molecular weight 

48kDa

Swiss Prot

Q16576

Gene ID (NCBI)

5931

Alternative Names

Histone binding protein RBBP7, RbAp46, RBBP 7, RBBP7

 

Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:100

IP

1:50 - 1:200

ChIP

1:50 - 1:200

 1                            2             

Western blot analysis of extracts of various cell lines, using RBBP7 antibody.


Immunofluorescence analysis of MCF-7 cells using RBBP7 antibody.

 

3   4             

Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug RBBP7 antibody.


Chromatin immunoprecipitation analysis extracts of 293T cells, using RBBP7 antibody.

     

Background


This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. 

                                                                                                 

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.