Product name | ATP6V1E1 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human ATP6V1E1 Calculated molecular weight 22kDa/23kDa/26kDa Observed molecular weight 35kDa Swiss Prot P36543 Gene ID (NCBI) 529 Alternative Names ARCL2C;ATP6E;ATP6E2;ATP6V1E;P31;Vma4 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:1000 - 1:2000 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |