欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

PAB33681 ATP5J Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB33681
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB33681 ATP5J Polyclonal Antibody

320.00元

Catalog : PAB33681
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information


Product name

ATP5J Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human ATP5J

Calculated molecular weight

12kDa/13kDa

Observed molecular weight 

13kDa

Swiss Prot

P18859

Gene ID (NCBI)

522

Alternative Names

ATP5;ATP5A;ATPM;CF6;F6


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:200 - 1:2000

IF

1:50 - 1:200

IHC

1:50 - 1:500

blob.png                   blob.png                  

Western blot analysis of extracts of various cell lines, using ATP5J antibody.


Immunofluorescence analysis of U2OS cells using ATP5J antibody.


Background

  

 Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.                                                                           


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.