Product name | ATP5J Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human ATP5J Calculated molecular weight 12kDa/13kDa Observed molecular weight 13kDa Swiss Prot P18859 Gene ID (NCBI) 522 Alternative Names ATP5;ATP5A;ATPM;CF6;F6 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:200 - 1:2000 IF 1:50 - 1:200 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using ATP5J antibody. Immunofluorescence analysis of U2OS cells using ATP5J antibody.
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
