Product name | RASGRF1 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human RASGRF1 Calculated molecular weight 55kDa/143kDa/145kDa Observed molecular weight 145kDa Swiss Prot Q13972 Gene ID (NCBI) 5923 Alternative Names CDC25;CDC25L;GNRP;GRF1;GRF55;H-GRF55;PP13187;ras-GRF1 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 Western blot analysis of extracts of mouse brain, using RASGRF1 antibody. Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody. Immunofluorescence analysis of U2OS cells using RASGRF1 antibody. 
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
