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PAB34933 RASGRF1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34933
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human,Mouse

库存 800 件

PAB34933 RASGRF1 Polyclonal Antibody

320.00元

Catalog : PAB34933
Application : WB,IHC,IF,ELISA
Reactivity : Human,Mouse
Product Information


Product name

RASGRF1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human,Mouse

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human RASGRF1

Calculated molecular weight

55kDa/143kDa/145kDa

Observed molecular weight 

145kDa

Swiss Prot

Q13972

Gene ID (NCBI)

5923

Alternative Names

CDC25;CDC25L;GNRP;GRF1;GRF55;H-GRF55;PP13187;ras-GRF1


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:100

 图片关键词      2      3                      

Western blot analysis of extracts of mouse brain, using RASGRF1 antibody.

Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody.

Immunofluorescence analysis of U2OS cells using RASGRF1 antibody.

 

Background


The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.                                                                                 

                                                                                                

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.