Product name | ACTN2 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human ACTN2 Calculated molecular weight 103kDa Observed molecular weight 115kDa Swiss Prot P35609 Gene ID (NCBI) 88 Alternative Names CMD1AA;CMH23 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IF 1:50 - 1:200 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using ACTN2 antibody.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |