Product name | HIST1H2BG Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human HIST1H2BG Calculated molecular weight 13kDa Observed molecular weight 14kDa Swiss Prot P62807 Gene ID (NCBI) 8339 Alternative Names dJ221C16.8;H2B.1A;H2B/a;H2BFA
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:100 - 1:200 |
Western blot analysis of extracts of various cell lines, using HIST1H2BG antibody. | Immunohistochemistry of paraffin-embedded mouse brain using HIST1H2BG antibody. |
Immunohistochemistry of paraffin-embedded human stomach using HIST1H2BG antibody. | Immunohistochemistry of paraffin-embedded rat brain using HIST1H2BG antibody. |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |