Product name | PSMD8 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human PSMD8 Calculated molecular weight 39kDa Observed molecular weight 30kDa, 35kDa Swiss Prot P48556 Gene ID (NCBI) 5714 Alternative Names HEL-S-91n;HIP6;HYPF;Nin1p;p31;Rpn12;S14 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 Western blot analysis of extracts of various cell lines, using PSMD8 antibody. Immunohistochemistry of paraffin-embedded human liver injury using PSMD8 antibody. Immunofluorescence analysis of U2OS cells using PSMD8 antibody. 
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
