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PAB34927 PSMD8 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34927
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human,Mouse

库存 800 件

PAB34927 PSMD8 Polyclonal Antibody

320.00元

Catalog : PAB34927
Application : WB,IHC,IF,ELISA
Reactivity : Human,Mouse
Product Information


Product name

PSMD8 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human,Mouse

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human PSMD8

Calculated molecular weight

39kDa

Observed molecular weight 

30kDa, 35kDa

Swiss Prot

P48556

Gene ID (NCBI)

5714

Alternative Names

HEL-S-91n;HIP6;HYPF;Nin1p;p31;Rpn12;S14


Application Notes

     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000



IHC

1:50 - 1:200

IF

1:50 - 1:100

 

    1                       

Western blot analysis of extracts of various cell lines, using PSMD8 antibody.

 

 2              3                  

Immunohistochemistry of paraffin-embedded human liver injury using PSMD8 antibody.


Immunofluorescence analysis of U2OS cells using PSMD8 antibody.

                              

Background

 

        The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.  An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.                                                                                               


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.