Product name | MAP2K5 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human MAP2K5 Calculated molecular weight 46kDa/48kDa/49kDa/50kDa Observed molecular weight 50kDa Swiss Prot Q13163 Gene ID (NCBI) 5607 Alternative Names HsT17454;MAPKK5;MEK5;PRKMK5 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 Western blot analysis of extracts of various cell lines, using MAP2K5 antibody. Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody. Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody. Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody. Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody. Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
