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PAB36309-P Phospho-RAF1-S621 pAb

价格:    580.00元

  • Catalog:    PAB36309-P
  • Application:    WB,IF,IP,ELISA,IHC
  • Reactivity:    Human

库存 800 件

PAB36309-P Phospho-RAF1-S621 pAb

580.00元

Catalog : PAB36309-P
Application : WB,IF,IP,ELISA,IHC
Reactivity : Human
Product Information

 

Product name

Phospho-RAF1-S621 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,IP,ELISA,IHC

Species reactivity

Human

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding S621 of human RAF1

Calculated molecular weight

73kDa/75kDa

Observed molecular weight 

73kDa

Swiss Prot

P04049

Gene ID (NCBI)

5894

Alternative Names

c-Raf;CMD1NN;CRAF;NS5;Raf-1


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IP

1:50 - 1:100

IHC

1:50 - 1:500

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Western blot analysis of extracts of HeLa and 293T cells, using Phospho-RAF1-S621 antibody.

Immunofluorescence analysis of U2OS cells using Phospho-RAF1-S621 antibody.

Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by EGF using 2.5ug Phospho-RAF1-S621 antibody.


Background


This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.   


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.