Lyophilized or Liquid
A phospho specific peptide corresponding to residues surrounding S621 of human RAF1
Calculated molecular weight
Observed molecular weight
Gene ID (NCBI)
1:500 - 1:2000
1:50 - 1:200
1:50 - 1:100
1:50 - 1:500
Western blot analysis of extracts of HeLa and 293T cells, using Phospho-RAF1-S621 antibody.
Immunofluorescence analysis of U2OS cells using Phospho-RAF1-S621 antibody.
Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by EGF using 2.5ug Phospho-RAF1-S621 antibody.
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.