欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

PAB36305-P Phospho-MDM2-S166 pAb

价格:    580.00元

  • Catalog:    PAB36305-P
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36305-P Phospho-MDM2-S166 pAb

580.00元

Catalog : PAB36305-P
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

Phospho-MDM2-S166 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification



Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding S166 of human MDM2

Calculated molecular weight

11-14kDa/24-55kDa

Observed molecular weight 

95kDa

Swiss Prot

Q00987

Gene ID (NCBI)

4193

Alternative Names

ACTFS;hdm2;HDMX


Application Notes




The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:1000

IF

1:50 - 1:100

IHC

1:50 - 1:500

  blob.png                                         blob.png                           

Western blot analysis of extracts of 293 cells, using Phospho-MDM2-S166 antibody.


Immunofluorescence analysis of U2OS cells using Phospho-MDM2-S166 antibody.



Background



This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.  



Storage



Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.