Product name | PPL Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human PPL Calculated molecular weight 204kDa Observed molecular weight 205kDa Swiss Prot O60437 Gene ID (NCBI) 5493 Alternative Names periplakin The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IF 1:50 - 1:100 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using PPL antibody. Immunofluorescence analysis of MCF7 cells using PPL antibody. 
The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
