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PAB34923 PPL Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34923
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB34923 PPL Polyclonal Antibody

320.00元

Catalog : PAB34923
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information


Product name

PPL Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human PPL

Calculated molecular weight

204kDa

Observed molecular weight 

205kDa

Swiss Prot

O60437

Gene ID (NCBI)

5493

Alternative Names

periplakin


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:100

IHC

1:50 - 1:500

     1                             2           

Western blot analysis of extracts of various cell lines, using PPL antibody.


Immunofluorescence analysis of MCF7 cells using PPL antibody.

                

Background

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.                       

                                                                                                 

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.