欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

PAB36298-P Phospho-MAP3K5-T918 pAb

价格:    580.00元

  • Catalog:    PAB36298-P
  • Application:    WB,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36298-P Phospho-MAP3K5-T918 pAb

580.00元

Catalog : PAB36298-P
Application : WB,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

Phospho-MAP3K5-T918 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification



Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding T918 of human MAP3K5

Calculated molecular weight

69kDa/154kDa

Observed molecular weight 

130kDa

Swiss Prot

Q99683

Gene ID (NCBI)

4217

Alternative Names

ASK1;MAPKKK5;MEKK5


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:500

           blob.png

Western blot analysis of extracts of HeLa cells, using Phospho-MAP3K5-T918 antibody.




Background



Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.  



Storage



Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.