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PAB36295-P Phospho-MAPK14-T180 pAb

价格:    580.00元

  • Catalog:    PAB36295-P
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Rat

库存 800 件

PAB36295-P Phospho-MAPK14-T180 pAb

580.00元

Catalog : PAB36295-P
Application : WB,IF,ELISA,IHC
Reactivity : Human,Rat
Product Information

 

Product name

Phospho-MAPK14-T180 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Rat

Host Species

Rabbit

Purity

Affinity purification



Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding T180 of human MAPK14

Calculated molecular weight

29kDa/34kDa/35kDa/41kDa

Observed molecular weight 

38kDa

Swiss Prot

Q16539

Gene ID (NCBI)

1432

Alternative Names

CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:200

IHC

1:50 - 1:500

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Western blot analysis of extracts of PC-12 cells, using Phospho-MAPK14-T180 antibody.


Immunofluorescence analysis of U2OS cells using Phospho-MAPK14-T180 antibody.



Background



The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.  



Storage



Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.