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PAB36294-PPhospho-MAPK14-Y322 pAb

价格:    580.00元

  • Catalog:    PAB36294-P
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse

库存 800 件

PAB36294-PPhospho-MAPK14-Y322 pAb

580.00元

Catalog : PAB36294-P
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse
Product Information

 

Product name

Phospho-MAPK14-Y322 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse

Host Species

Rabbit

Purity

Affinity purification

 

Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding Y322 of human MAPK14

Calculated molecular weight

29kDa/34kDa/35kDa/41kDa

Observed molecular weight 

41kDa

Swiss Prot

Q16539

Gene ID (NCBI)

1432

Alternative Names

CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A



Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:200

IHC

1:50 - 1:500

        blob.png                   blob.png

Western blot analysis of extracts of 293 and NIH/3T3 cells, using Phospho-MAPK14-Y322 antibody.


Immunofluorescence analysis of U2OS cells using Phospho-MAPK14-Y322 antibody.


Background


The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.   


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.