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PAB34920 PMS2 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34920
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB34920 PMS2 Polyclonal Antibody

320.00元

Catalog : PAB34920
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information


Product name

PMS2 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human PMS2

Calculated molecular weight

20kDa/51kDa/62kDa/95kDa

Observed molecular weight 

96kDa, 115kDa

Swiss Prot

P54278

Gene ID (NCBI)

5395

Alternative Names

HNPCC4;MLH4;PMS2CL;PMSL2


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:100

IHC

1:50 - 1:500

 

    1                       

Western blot analysis of extracts of various cell lines, using PMS2 antibody.

 

     2              

Immunofluorescence analysis of A-549 cells using PMS2 antibody.     


Background


The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.                                                                                        

                                                                                                  

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.