Product name | PLOD2 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human PLOD2 Calculated molecular weight 49kDa/84kDa/87kDa Observed molecular weight 84kDa Swiss Prot O00469 Gene ID (NCBI) 5352 Alternative Names BRKS2;LH2;TLH The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IF 1:50 - 1:100 IHC 1:50 - 1:500 Western blot analysis of extracts of rat kidney, using PLOD2 Antibody. Immunofluorescence analysis of U2OS cells using PLOD2 antibody.
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |