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PAB36185 RPE65 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB36185
  • Application:    WB,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36185 RPE65 Polyclonal Antibody

320.00元

Catalog : PAB36185
Application : WB,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

RPE65 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human RPE65

Calculated molecular weight

60kDa

Observed molecular weight 

61kDa

Swiss Prot

Q16518

Gene ID (NCBI)

6121

Alternative Names

BCO3;LCA2;mRPE65;rd12;RP20;sRPE65


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:500

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Western blot analysis of extracts of various cell lines, using RPE65 antibody.   


Background


This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.  


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.