Product name | Phospho-ERK3-S189 pAb |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | A phospho specific peptide corresponding to residues surrounding S189 of human ERK3 |
Calculated molecular weight | 82kDa |
Observed molecular weight | 120kDa |
Swiss Prot | Q16659 |
Gene ID (NCBI) | 5597 |
Alternative Names | ERK3;HsT17250;p97MAPK;PRKM6 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |