Product name | PARN Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human PARN Calculated molecular weight 52kDa/66kDa/67kDa/73kDa Observed molecular weight 73kDa Swiss Prot O95453 Gene ID (NCBI) 5073 Alternative Names DAN;DKCB6;PFBMFT4 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IF 1:50 - 1:100 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using PARN antibody. Immunofluorescence analysis of MCF7 cells using PARN antibody.
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |