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PAB35587 PRKAB1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35587
  • Application:    WB,IHC,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35587 PRKAB1 Polyclonal Antibody

320.00元

Catalog : PAB35587
Application : WB,IHC,ELISA
Reactivity : Human,Mouse,Rat
Product Information


Product name

PRKAB1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic peptide of human PRKAB1

Calculated molecular weight

30kDa

Observed molecular weight 

37kDa

Swiss Prot

Q9Y478

Gene ID (NCBI)

5564

Alternative Names

AMPK;HAMPKb


Application Notes


The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

 

   1                     2             

Western blot analysis of extracts of various cell lines, using PRKAB1 antibody.


Immunohistochemistry of paraffin-embedded rat spleen using PRKAB1 antibody.


 3                4               

Immunohistochemistry of paraffin-embedded rat kidney using PRKAB1 antibody.


Immunohistochemistry of paraffin-embedded mouse kidney using PRKAB1 antibody.

     

Background


The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.           

                                                                                                 

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.