Product name | NEDD4L Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human NEDD4L Calculated molecular weight 96-111kDa Observed molecular weight 113kDa Swiss Prot Q96PU5 Gene ID (NCBI) 23327 Alternative Names hNEDD4-2;NEDD4-2;NEDD4.2;PVNH7;RSP5;hNEDD4-2;NEDD4-2;NEDD4.2;RSP5 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 Western blot analysis of extracts of various cell lines, using NEDD4L antibody. Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody. Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody. Immunofluorescence analysis of U2OS cells using NEDD4L antibody.
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |