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PAB35683 NEDD4L Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35683
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35683 NEDD4L Polyclonal Antibody

320.00元

Catalog : PAB35683
Application : WB,IHC,IF,ELISA
Reactivity : Human,Mouse,Rat
Product Information


Product name

NEDD4L Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human NEDD4L

Calculated molecular weight

96-111kDa

Observed molecular weight 

113kDa

Swiss Prot

Q96PU5

Gene ID (NCBI)

23327

Alternative Names

hNEDD4-2;NEDD4-2;NEDD4.2;PVNH7;RSP5;hNEDD4-2;NEDD4-2;NEDD4.2;RSP5


Application Notes


   

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

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Western blot analysis of extracts of various cell lines, using NEDD4L antibody.


Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody.

 

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Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody.


Immunofluorescence analysis of U2OS cells using NEDD4L antibody.


Background

  

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.                                                                            


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.