Product name | POU5F1 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen A synthetic peptide of human POU5F1 Calculated molecular weight 30kDa/38kDa Observed molecular weight 50kDa Swiss Prot Q01860 Gene ID (NCBI) 5460 Alternative Names Oct-3;Oct-4;OCT3;OCT4;OTF-3;OTF3;OTF4 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 Western blot analysis of extracts of various cell lines, using POU5F1 antibody. Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody. Immunofluorescence analysis of U2OS cells using POU5F1 antibody. 
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
