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PAB35586 POU5F1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35586
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35586 POU5F1 Polyclonal Antibody

320.00元

Catalog : PAB35586
Application : WB,IHC,IF,ELISA
Reactivity : Human,Mouse,Rat
Product Information


Product name

POU5F1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic peptide of human POU5F1

Calculated molecular weight

30kDa/38kDa

Observed molecular weight 

50kDa

Swiss Prot

Q01860

Gene ID (NCBI)

5460

Alternative Names

Oct-3;Oct-4;OCT3;OCT4;OTF-3;OTF3;OTF4


Application Notes


The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

 

   1                        

Western blot analysis of extracts of various cell lines, using POU5F1 antibody.

 

 2                        3              

Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody.


Immunofluorescence analysis of U2OS cells using POU5F1 antibody.

     

Background


This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.                                                                                                         


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.