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PAB36175 LAMB1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB36175
  • Application:    WB,IHC,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36175 LAMB1 Polyclonal Antibody

320.00元

Catalog : PAB36175
Application : WB,IHC,ELISA
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

LAMB1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human LAMB1

Calculated molecular weight

198kDa

Observed molecular weight 

198kDa

Swiss Prot

P07942

Gene ID (NCBI)

3912

Alternative Names

CLM, LAMB1, Laminin 1 subunit beta, Laminin 10 subunit beta, Laminin 12 subunit beta, Laminin 2 subunit beta, Laminin 6 subunit beta, Laminin 8 subunit beta, Laminin B1 chain, Laminin subunit beta 1, laminin, beta 1


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:1000 - 1:4000

IHC

1:100 - 1:200

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Western blot analysis of extracts of various cell lines, using LAMB1 antibody.


Background


Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

 

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.