Product name | PLOD1 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human PLOD1 Calculated molecular weight 83kDa/88kDa Observed molecular weight 110kDa Swiss Prot Q02809 Gene ID (NCBI) 5351 Alternative Names EDS6;LH;LH1;LLH;PLOD The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IF 1:50 - 1:200 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using PLOD1 antibody. Immunofluorescence analysis of U2OS cells using PLOD1 antibody.
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |