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PAB35585 PLOD1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35585
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse

库存 800 件

PAB35585 PLOD1 Polyclonal Antibody

320.00元

Catalog : PAB35585
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse
Product Information


Product name

PLOD1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human PLOD1

Calculated molecular weight

83kDa/88kDa

Observed molecular weight 

110kDa

Swiss Prot

Q02809

Gene ID (NCBI)

5351

Alternative Names

EDS6;LH;LH1;LLH;PLOD


Application Notes


  

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:200

IHC

1:50 - 1:500

 

     1                      

Western blot analysis of extracts of various cell lines, using PLOD1 antibody.

 

     2              

Immunofluorescence analysis of U2OS cells using PLOD1 antibody.



   

Background


Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.                                                                                                        

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.