Product name | Phospho-FOS-T232 pAb |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | A phospho specific peptide corresponding to residues surrounding T232 of human FOS |
Calculated molecular weight | 28kDa/36kDa/40kDa |
Observed molecular weight | 62kDa |
Swiss Prot | P01100 |
Gene ID (NCBI) | 2353 |
Alternative Names | AP-1;C-FOS;p55 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IF | 1:50 - 1:200 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of HepG2 cells, using Phospho-FOS-T232 antibody. | Immunofluorescence analysis of MCF-7 cells using Phospho-FOS-T232 antibody. |
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |