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PAB34910 AFF1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB34910
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human

库存 800 件

PAB34910 AFF1 Polyclonal Antibody

320.00元

Catalog : PAB34910
Application : WB,IHC,IF,ELISA
Reactivity : Human
Product Information

Product name

AFF1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human AFF1

Calculated molecular weight

131kDa/132kDa

Observed molecular weight 

Refer to figures

Swiss Prot

P51825

Gene ID (NCBI)

4299

Alternative Names

AF4;MLLT2;PBM1


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:100

 

    1                       

Immunofluorescence analysis of U2OS cells using AFF1 antibody.

 

Background


This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.                                                                                                       

                                                                                                 

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.