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PAB35667 RFC5 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35667
  • Application:    WB,IHC,ELISA
  • Reactivity:    Human,Mouse

库存 800 件

PAB35667 RFC5 Polyclonal Antibody

320.00元

Catalog : PAB35667
Application : WB,IHC,ELISA
Reactivity : Human,Mouse
Product Information


Product name

RFC5 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,ELISA

Species reactivity

Human,Mouse

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human RFC5

Calculated molecular weight

36kDa/38kDa

Observed molecular weight 

38kDa

Swiss Prot

P40937

Gene ID (NCBI)

5985

Alternative Names

Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5


Application Notes


     

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

 

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Western blot analysis of extracts of various cell lines, using RFC5 antibody.


Immunohistochemistry of paraffin-embedded human lung cancer using RFC5 antibody.


Background


 The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.                                                                          

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.