RFC5 Polyclonal Antibody
Lyophilized or Liquid
Recombinant protein of human RFC5
Calculated molecular weight
Observed molecular weight
Gene ID (NCBI)
Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5
1:500 - 1:2000
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RFC5 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using RFC5 antibody.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.