Product name | Phospho-EIF4EBP1-T70 pAb |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | A phospho specific peptide corresponding to residues surrounding T70 of human EIF4EBP1 |
Calculated molecular weight | 12kDa |
Observed molecular weight | 20kDa |
Swiss Prot | Q13541 |
Gene ID (NCBI) | 1978 |
Alternative Names | 4E-BP1;4EBP1;BP-1;PHAS-I |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of 293 cells, using Phospho-EIF4EBP1-T70 antibody.
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
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文献链接:https://www.frontiersin.org/articles/10.3389/fmolb.2021.645365/full
文献题目:Long Non-coding RNA DLEU2L Targets miR-210-3p to Suppress Gemcitabine Resistance in Pancreatic Cancer Cells via BRCA2 Regulation
发表期刊:Frontiers in Molecular Biosciences
影响因子:5.246
DOI:10.3389/fmolb.2021.645365
出版信息:Front Mol Biosci. 2021 Apr;0:296