Product name | Phospho-ATF2-T71 pAb |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human,Mouse |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | A phospho specific peptide corresponding to residues surrounding T71 of human ATF2 |
Calculated molecular weight | 13- 15kDa/23-24kDa/35-54kDa |
Observed molecular weight | 70kDa |
Swiss Prot | P15336 |
Gene ID (NCBI) | 1386 |
Alternative Names | HB16; CREB2; TREB7; CREB-2; CRE-BP1 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:1000 |
IF | 1:50 - 1:100 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of various cell lines, using Phospho-ATF2-T71 antibody. | Immunofluorescence analysis of MCF-7 cells using Phospho-ATF2-T71 antibody. |
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
