Product name | Phospho-CREB1-S133 pAb |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IF,ELISA,IHC |
Species reactivity | Human |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | A phospho specific peptide corresponding to residues surrounding Ser133 of human CREB |
Calculated molecular weight | 25kDa/35kDa/36kDa |
Observed molecular weight | 43kDa |
Swiss Prot | P16220 |
Gene ID (NCBI) | 1385 |
Alternative Names | CREB;CREB-1 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:200 - 1:2000 |
IF | 1:50 - 1:100 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 antibody. | Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 antibody. |
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
