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PAB36276-P Phospho-CDK1-Y15 pAb

价格:    580.00元

  • Catalog:    PAB36276-P
  • Application:    WB,IF,IP,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36276-P Phospho-CDK1-Y15 pAb

580.00元

Catalog : PAB36276-P
Application : WB,IF,IP,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

Phospho-CDK1-Y15 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,IP,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A phospho specific peptide corresponding to residues surrounding Y15 of human CDK1

Calculated molecular weight

27kDa/34kDa

Observed molecular weight 

34kDa

Swiss Prot

P06493

Gene ID (NCBI)

983

Alternative Names

CDC2;CDC28A;P34CDC2


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:1000

IF

1:50 - 1:100

IP

1:50 - 1:100

IHC

1:50 - 1:500

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Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-Y15 antibody.

Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-Y15 antibody.

Immunoprecipitation analysis of 200ug extracts of HT-29 cells treated by starvation using 2.5ug Phospho-CDK1-Y15 antibody.

Background


The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.