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PAB35571 HARS Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35571
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35571 HARS Polyclonal Antibody

320.00元

Catalog : PAB35571
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information


Product name

HARS Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human HARS

Calculated molecular weight

50kDa/52kDa/54kDa/57kDa

Observed molecular weight 

57kDa

Swiss Prot

P12081

Gene ID (NCBI)

3035

Alternative Names

CMT2W;HRS;USH3B


Application Notes


  

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IF

1:50 - 1:100

IHC

1:50 - 1:500

1             2                        

Western blot analysis of extracts of various cell lines, using HARS antibody.


Immunofluorescence analysis of A549 cells using HARS antibody.

   

Background


Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.                                                                                                            


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.