The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Western blot analysis of extracts of various cell lines, using SARDH Antibody.

This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described.