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PAB36270 GAPDH Monoclonal Antibody

价格:    320.00元

  • Catalog:    PAB36270
  • Application:    WB,IHC,IF,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB36270 GAPDH Monoclonal Antibody

320.00元

Catalog : PAB36270
Application : WB,IHC,IF,ELISA
Reactivity : Human,Mouse,Rat
Product Information

 

Product name

GAPDH Monoclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Mouse

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human GAPDH

Calculated molecular weight

31kDa/36kDa

Observed molecular weight 

36kDa

Swiss Prot

P04406

Gene ID (NCBI)

2597

Alternative Names

G3PD;GAPD;HEL-S-162eP


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:50000 - 1:200000

IHC

1:50 - 1:200

IF

1:50 - 1:200

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Western blot analysis of extracts of various cell lines, using GAPDH antibody.


Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody.

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Immunohistochemistry of paraffin-embedded rat heart using GAPDH antibody.


Immunohistochemistry of paraffin-embedded human tonsil using GAPDH antibody.

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Immunohistochemistry of paraffin-embedded human lung cancer using GAPDH antibody.


Immunohistochemistry of paraffin-embedded human colon using GAPDH antibody.

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Immunohistochemistry of paraffin-embedded mouse spinal cord using GAPDH antibody.


Immunofluorescence analysis of PC-12 cells using GAPDH antibody.


Background


This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. 


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.