Product name | GAPDH Monoclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,IF,ELISA |
Species reactivity | Human,Mouse,Rat |
Host Species | Mouse |
Purity | Affinity purification |
Immunogen | Recombinant protein of human GAPDH |
Calculated molecular weight | 31kDa/36kDa |
Observed molecular weight | 36kDa |
Swiss Prot | P04406 |
Gene ID (NCBI) | 2597 |
Alternative Names | G3PD;GAPD;HEL-S-162eP |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:50000 - 1:200000 |
IHC | 1:50 - 1:200 |
IF | 1:50 - 1:200 |
Western blot analysis of extracts of various cell lines, using GAPDH antibody. | Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody. |
Immunohistochemistry of paraffin-embedded rat heart using GAPDH antibody. | Immunohistochemistry of paraffin-embedded human tonsil using GAPDH antibody. |
Immunohistochemistry of paraffin-embedded human lung cancer using GAPDH antibody. | Immunohistochemistry of paraffin-embedded human colon using GAPDH antibody. |
Immunohistochemistry of paraffin-embedded mouse spinal cord using GAPDH antibody. | Immunofluorescence analysis of PC-12 cells using GAPDH antibody. |
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |