GAPDH Polyclonal Antibody
Lyophilized or Liquid
Immunogen Recombinant protein of human GAPDH Calculated molecular weight 31kDa/36kDa Observed molecular weight 36kDa Swiss Prot P04406 Gene ID (NCBI) 2597 Alternative Names G3PD;GAPD;HEL-S-162eP
Recombinant protein of human GAPDH
Calculated molecular weight
Observed molecular weight
Gene ID (NCBI)
1:1000 - 1:2000
1:50 - 1:500
Western blot analysis of extracts of various cell lines, using GAPDH antibody.
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.