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PAB35545 ACO1 Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35545
  • Application:    WB,IHC,ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35545 ACO1 Polyclonal Antibody

320.00元

Catalog : PAB35545
Application : WB,IHC,ELISA
Reactivity : Human,Mouse,Rat
Product Information


Product name

ACO1 Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human ACO1

Calculated molecular weight

98kDa

Observed molecular weight 

120kDa

Swiss Prot

P21399

Gene ID (NCBI)

48

Alternative Names

ACONS;HEL60;IREB1;IREBP;IREBP1;IRP1


Application Notes


 

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:100

 

  1                         

Western blot analysis of extracts of various cell lines, using ACO1 antibody.

    2                      3

Immunohistochemistry of paraffin-embedded human prostate using ACO1 antibody.


Immunohistochemistry of paraffin-embedded rat kidney using ACO1 antibody.

    

Background


The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.                                                                                                                                           


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.