Product name | NEFH Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,ELISA |
Species reactivity | Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | Recombinant protein of human NEFH |
Calculated molecular weight | 105kDa/112kDa |
Observed molecular weight | 200kDa |
Swiss Prot | P12036 |
Gene ID (NCBI) | 4744 |
Alternative Names | CMT2CC;NFH |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:100 |
Western blot analysis of extracts of mouse brain, using NEFH antibody. | Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody. |
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody. | Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody. |
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |