Product name | HLA-E Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen A synthetic peptide of human HLA-E Calculated molecular weight 40kDa Observed molecular weight 45kDa Swiss Prot P13747 Gene ID (NCBI) 3133 Alternative Names EA1.2;EA2.1;HLA-6.2;MHC;QA1;HLA-6.2;QA1 The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:500 Western blot analysis of extracts of various cell lines, using HLA-E antibody
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
