Product name | SIRPA Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse,Rat |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human SIRPA Calculated molecular weight 54kDa/55kDa Observed molecular weight 120kDa Swiss Prot P78324 Gene ID (NCBI) 140885 Alternative Names BIT;CD172A;MFR;MYD-1;P84;PTPNS1;SHPS1;SIRP
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:200 - 1:2000 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of various cell lines, using SIRPA antibody.
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
