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PAB35527 ATG4D Polyclonal Antibody

价格:    320.00元

  • Catalog:    PAB35527
  • Application:    WB,IF,ELISA,IHC
  • Reactivity:    Human,Mouse,Rat

库存 800 件

PAB35527 ATG4D Polyclonal Antibody

320.00元

Catalog : PAB35527
Application : WB,IF,ELISA,IHC
Reactivity : Human,Mouse,Rat
Product Information


Product name

ATG4D Polyclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IF,ELISA,IHC

Species reactivity

Human,Mouse,Rat

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

Recombinant protein of human ATG4D

Calculated molecular weight

16kDa/52kDa

Observed molecular weight 

53kDa

Swiss Prot

Q86TL0

Gene ID (NCBI)

84971

Alternative Names

APG4-D;APG4D;AUTL4


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

WB 1:500 - 1:2000

IF

1:50 - 1:200

IHC 1:50 - 1:200

IHC

1:50 - 1:500


 1             2                       

Western blot analysis of extracts of various cell lines, using ATG4D antibody.


Immunofluorescence analysis of U2OS cells using ATG4D antibody.

     

Background


 Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.                                                                                                   


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.