Product name | ALAD Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,IHC,ELISA |
Species reactivity | Human |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen Recombinant protein of human ALAD Calculated molecular weight 36kDa/39kDa Observed molecular weight 36kDa Swiss Prot P13716 Gene ID (NCBI) 210 Alternative Names ALADH;PBGS The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1:500 - 1:2000 IHC 1:50 - 1:100 Western blot analysis of extracts of various cell lines, using ALAD antibody. Immunohistochemistry of paraffin-embedded human stomach using ALAD antibody.
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
