The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Western blot analysis of extracts of various cell lines, using MIB1 antibody.

This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).