Product name | RGS14 Polyclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB,ELISA,IHC |
Species reactivity | Human,Mouse |
Host Species | Rabbit |
Purity | Affinity purification |
Immunogen | Recombinant protein of human RGS14 |
Calculated molecular weight | 21kDa/36kDa/44kDa/61kDa |
Observed molecular weight | 54kDa |
Swiss Prot | O43566 |
Gene ID (NCBI) | 10636 |
Alternative Names | regulator of G-protein signaling 14 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:500 |
Western blot analysis of extracts of various cell lines, using RGS14 antibody.
This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |