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PAB33322 MonoMethyl-Histone H4-K20 pAb

价格:    440.00元

  • Catalog:    PAB33322
  • Application:    WB,IHC,IF,IP,ChIP,ChIPseq,ELISA
  • Reactivity:    Human,Mouse,Rat,Other (Wide Range)

库存 800 件

PAB33322 MonoMethyl-Histone H4-K20 pAb

440.00元

Catalog : PAB33322
Application : WB,IHC,IF,IP,ChIP,ChIPseq,ELISA
Reactivity : Human,Mouse,Rat,Other (Wide Range)
Product Information


Product name

MonoMethyl-Histone H4-K20 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,IP,ChIP,ChIPseq,ELISA

Species reactivity

Human,Mouse,Rat,Other (Wide Range)

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic peptide of human MonoMethyl-Histone H4-K20

Calculated molecular weight

11kDa

Observed molecular weight 

13kDa

Swiss Prot

P62805

Gene ID (NCBI)

8370

Alternative Names

FO108;H4;H4/n;H4F2;H4FN;HIST2H4


Application Notes


  

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

IP

1:50 - 1:200

ChIP

1:20 - 1:100

CHIPseq

1:20 - 1:100

         blob.png           blob.png       

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-K20 antibody.


Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H4-K20 antibody.

 

blob.png                   Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 antibody.     

   

Background

     Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.                                                                                             

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.