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PAB33312 TriMethyl-Histone H3-K9 pAb

价格:    440.00元

  • Catalog:    PAB33312
  • Application:    WB,IHC,IF,IP,ChIP,ChIPseq
  • Reactivity:    Human,Mouse,Rat,Other (Wide Range)

库存 800 件

PAB33312 TriMethyl-Histone H3-K9 pAb

440.00元

Catalog : PAB33312
Application : WB,IHC,IF,IP,ChIP,ChIPseq
Reactivity : Human,Mouse,Rat,Other (Wide Range)
Product Information


Product name

TriMethyl-Histone H3-K9 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,IP,ChIP,ChIPseq

Species reactivity

Human,Mouse,Rat,Other (Wide Range)

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3

Calculated molecular weight

15kDa

Observed molecular weight 

17kDa

Swiss Prot

Q16695

Gene ID (NCBI)

8290

Alternative Names

H3.4;H3/g;H3FT;H3t


Application Notes


  

determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

IP

1:50 - 1:200

ChIP

1:50 - 1:200

CHIPseq

1:50 - 1:200

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody.


Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.

 

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Immunohistochemistry of paraffin-embedded rat lung using TriMethyl-Histone H3-K9 antibody.

Immunohistochemistry of paraffin-embedded human kidney using TriMethyl-Histone H3-K9 antibody.

Immunohistochemistry of paraffin-embedded human lung cancer using TriMethyl-Histone H3-K9 antibody.

    

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Immunohistochemistry of paraffin-embedded human liver cancer using TriMethyl-Histone H3-K9 antibody.

Immunohistochemistry of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K9 antibody.

Immunohistochemistry of paraffin-embedded Mouse lung using TriMethyl-Histone H3-K9 antibody.

 

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Immunohistochemistry of paraffin-embedded Rat brain using TriMethyl-Histone H3-K9 antibody.


Immunohistochemistry of paraffin-embedded Rat liver using TriMethyl-Histone H3-K9 antibody.

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K9 antibody.

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Chromatin immunoprecipitation analysis extracts of 293T cells, using TriMethyl-Histone H3-K9 antibody.

   

Background

   

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.   

                                                                                             

Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.