欢迎来到Bioswamp武汉贝茵莱生物科技有限公司   注册   登录

MU30369小鼠白细胞介素1β(IL-1β) Mouse Interleukin 1β(IL-1β)ELISA Kit

价格:    2400.00元

  • Catalog:    MU30369
96T

库存 100 件

MU30369小鼠白细胞介素1β(IL-1β) Mouse Interleukin 1β(IL-1β)ELISA Kit

2400.00元

Catalog : MU30369
用途

本试剂盒用于体外定量检测小鼠血清、血浆、组织、细胞上清及相关液体样本中白细胞介素1β(IL-1 β)的含量。

别称:

IL1b; IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin


检测原理

本试剂盒应用双抗体夹心法测定标本中小鼠白细胞介素1β(IL-1β)水平。用纯化的小鼠白细 胞介素1β(IL-1β)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素1 β(IL-1β),再与HRP标记的白细胞介素1β(IL-1β)抗体结合,形成抗体-抗原-酶标抗体复合 物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终 的黄色。颜色的深浅和样品中的白细胞介素1β(IL-1β)呈正相关。用酶标仪在450nm波长下测定 吸光度(OD值),通过标准曲线计算样品中小鼠白细胞介素1β(IL-1β)浓度。

 

反应类型

夹心法

反应时间

2h

反应

小鼠 

检测方法

Colormetric

检测范围

12pg/ml-960pg/ml

灵敏度

≤2.4pg/m

样本体积

50μL

样本类型

血清、血浆或其他相关生物液体


特异性

可检测样本中的小鼠白细胞介素1β(IL-1β),且与其它相关蛋白无明显交叉反应


重复性

板内变异系数≤9%,板间变异系数≤11%


试剂盒组成及保存
规格
96T

说明书

1份

封板膜

2张

酶标包被板

12孔×8条

标准 1920pg/ml

0.6ml×1 瓶

标准品稀释液

2ml×1 瓶

生物素标记的抗IL-1β抗体

1.5ml×1瓶

显色剂A液

6ml×1瓶

显色剂B液

6ml×1瓶

终止液

6ml×1瓶

酶标试剂

6ml×1瓶

浓缩洗涤液

20ml×1瓶(30倍)


保存温度:2-8°C 

 

实验室所需物品

需要而未提供的试剂和器材

1.吸光度值在450nm波长下检测的酶标仪

2.1-2ml的加样器

3.100 ml和1L的量筒

4.吸水纸

5.37°C 恒温箱

6.蒸馏水或去离子水

7.数据分析和绘图软件,图纸

8.标准和样品稀释的试管‍

 

操作步骤


 

1529560202828115.jpg



参考文献



1、

文献链接:https://www.mdpi.com/1422-0067/22/22/12338

文献题目:An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia

发表期刊:International Journal Of Molecular Sciences

影响因子(IF):5.923

DOI:10.3390/ijms222212338

出版信息:Int J Mol Sci. 2021 Jan;22(22):12338


2、

文献链接:https://www.dovepress.com/clenbuterol-a-selective-2-adrenergic-receptor-agonist-inhibits-or-limi-peer-reviewed-fulltext-article-JIR

文献题目:Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice

发表期刊:Journal of Inflammation Research

影响因子(IF):6.922

DOI:10.2147/JIR.S344521

出版信息:J Inflamm Res. 2022 Jan;15:295-309


3、

图片关键词Journal of Nanobiotechnology补充材料.pdf


4、

文献链接:https://www.sciencedirect.com/science/article/pii/S0144861721009693

文献题目:Synthesis of branched β-1,3-glucan oligosaccharide with narrow degree of polymerization by fungi co-cultivation

发表期刊:Carbohydrate Polymers

影响因子:9.381

DOI10.1016/j.carbpol.2021.118582

出版信息:Carbohyd Polym. 2021 Dec;273:118582


5、

文献链接:https://www.sciencedirect.com/science/article/pii/S1385894719304498

文献题目:A novel chitosan-based nanomedicine for multi-drug resistant breast cancer therapy

发表期刊:Chemical Engineering Journal

影响因子:8.355

DOI10.1016/j.cej.2019.02.201

出版信息:Chem Eng J. 2019 Aug;369:134


6、

文献链接:https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-021-01227-1

文献题目:IL-17F depletion accelerates chitosan conduit guided peripheral nerve regeneration

发表期刊:Acta Neuropathologica Communications

影响因子:7.801

DOI10.1186/s40478-021-01227-1

出版信息:Acta Neuropathol Com. 2021 Dec;9(1):1-14


7、

文献链接:https://www.sciencedirect.com/science/article/pii/S0896841120301931

文献题目:Resistant starch intake alleviates collagen-induced arthritis in mice by modulating gut microbiota and promoting concomitant propionate production

发表期刊:Journal Of Autoimmunity

影响因子:6.658

DOI10.1016/j.jaut.2020.102564

出版信息:J Autoimmun. 2021 Jan;116:102564


8、

文献链接:https://www.sciencedirect.com/science/article/pii/S0889159118308055

文献题目:CXCL13/CXCR5 signaling contributes to diabetes-induced tactile allodynia via activating pERK, pSTAT3, pAKT pathways and pro-inflammatory cytokines production in the spinal cord of male mice

发表期刊:Brain Behavior And Immunity

影响因子:6.17

DOI10.1016/j.bbi.2019.05.020

出版信息:2019 May 14


9、

文献链接:https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7191

文献题目:Emodin alleviates LPS-induced myocardial injury through inhibition of NLRP3 inflammasome activation

发表期刊:Phytotherapy Research

影响因子:5.878

DOI10.1002/ptr.7191

出版信息:Phytother Res. 2021 Sep;35(9):5203-5213


10、

文献链接:https://www.frontiersin.org/articles/10.3389/fmicb.2021.692832/full

文献题目:Notch Signaling Ligand Jagged1 Enhances Macrophage-Mediated Response to Helicobacter pylori

发表期刊:Frontiers in Microbiology

影响因子:5.64

DOI10.3389/fmicb.2021.692832

出版信息:Front Microbiol. 2021 Jul;0:1741


11、

文献链接:https://cmjournal.biomedcentral.com/articles/10.1186/s13020-020-00322-0

文献题目:Anti-inflammatory properties of uvaol on DSS-induced colitis and LPS-stimulated macrophages

发表期刊:Chinese Medicine

影响因子:5.455

DOI10.1186/s13020-020-00322-0

出版信息:Chin Med-Uk. 2020 Dec;15(1):1-13


12、

文献链接:https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00493-4

文献题目:Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation

发表期刊:Chinese Medicine

影响因子:5.455

DOI10.1186/s13020-021-00493-4

出版信息:Chin Med-Uk. 2021 Dec;16(1):1-17


13、

文献链接:https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16654

文献题目:Bone marrow mesenchymal stem cellderived exosomal miR-206 promotes osteoblast proliferation and differentiation in osteoarthritis by reducing Elf3

发表期刊:Journal Of Cellular And Molecular Medicine

影响因子:5.31

DOI10.1111/jcmm.16654

出版信息:J Cell Mol Med. 2021 Aug;25(16):7734-7745


14、

文献链接:https://www.frontiersin.org/articles/10.3389/fimmu.2020.570920/full

文献题目:BAFF Blockade Attenuates Inflammatory Responses and Intestinal Barrier Dysfunction in a Murine Endotoxemia Model

发表期刊:Frontiers in Immunology

影响因子:5.085

DOI10.3389/fimmu.2020.570920

出版信息:2020 Nov 26


15、

文献链接:https://www.sciencedirect.com/science/article/pii/S1567576921006743

文献题目:Activation of CB2 receptor inhibits pyroptosis and subsequently ameliorates cecal ligation and puncture-induced sepsis

发表期刊:International Immunopharmacology

影响因子:4.932

DOI10.1016/j.intimp.2021.108038

出版信息:Int Immunopharmacol. 2021 Oct;99:108038