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MAB46145 HRP-conjugated GAPDH Monoclonal Antibody

价格:    440.00元

  • Catalog:    MAB46145

库存 600 件

MAB46145 HRP-conjugated GAPDH Monoclonal Antibody

440.00元

Catalog : MAB46145
Product Information


Product name

HRP-conjugated GAPDH Monoclonal Antibody

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB

Species reactivity

Mouse, Rat, Human, Fish (predicted: Horse, Chicken, Guinea pig, Hamster, Cat, Dog, Pig, Xenopus laevis, Monkey, Zebrafish, Baboon, Xenopus tropicalis)

Host Species

Mouse

Purity

Affinity purification



Immunogen Information


Immunogen

Recombinant protein of human GAPDH

Calculated molecular weight

36kDa

Observed molecular weight 

36kDa

Swiss Prot

P04406

Gene ID (NCBI)

2597

Alternative Names

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12


Application Notes




The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:5000 - 1:15000

   

         image.png          

Western blot analysis of GAPDH in various tissues and cell lines using HRP-Conjugated GAPDH Monoclonal Antibody at a dilution of 110000



                


Background



This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. 

 


Storage



Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.