Product name | HRP-conjugated GAPDH Monoclonal Antibody |
Isotype | IgG |
Form | Lyophilized or Liquid |
Tested applications | WB |
Species reactivity | Mouse, Rat, Human, Fish (predicted: Horse, Chicken, Guinea pig, Hamster, Cat, Dog, Pig, Xenopus laevis, Monkey, Zebrafish, Baboon, Xenopus tropicalis) |
Host Species | Mouse |
Purity | Affinity purification |
Immunogen | Recombinant protein of human GAPDH |
Calculated molecular weight | 36kDa |
Observed molecular weight | 36kDa |
Swiss Prot | P04406 |
Gene ID (NCBI) | 2597 |
Alternative Names | G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12 |
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB | 1:5000 - 1:15000 |
Western blot analysis of GAPDH in various tissues and cell lines using HRP-Conjugated GAPDH Monoclonal Antibody at a dilution of 1:10000 |
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
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文献链接:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334483/
文献题目:Magnetically Directed Enzyme/Prodrug Prostate Cancer Therapy Based on β-Glucosidase/Amygdalin
发表期刊:International Journal of Nanomedicine
影响因子:5.115
DOI:10.2147/IJN.S242359
出版信息:Int J Nanomed. 2020; 15: 4639–4657